Chocolate industry side streams as a valuable feedstock for microbial long-chain dicarboxylic acid production

Two high-fat containing side streams from the chocolate industry were evaluated as an alternative renewable substrate for biochemical long-chain dicarboxylic acid (DCA) production by Candida tropicalis. Cleaning water liquid transport and dissolved air flotation (DAF) sludge wastewater treatment plant contained 29 18 m% fat respectively. The addition of up to 100 g.L−1 ‘chocolate water’ 80 % (v/v) DAF had no negative effects on both growth DCA production. In a fed-batch reactor, concentration 5.8 was obtained after 120 h 47.5 consumed fats converted upon glucose when using water. Using sludge, 4.9 72 h, equalling 37.5 fats. This research shows potential researched